Selection of Pru p 3 hypoallergenic peach and nectarine varieties
نویسندگان
چکیده
منابع مشابه
Development of a hypoallergenic and immunogenic Pru p 3 proline variant for treatment of peach allergy
Methods We deployed an in-silico mutagenesis approach to design a fold variant of recombinant Pru p 3. Four stability hot spot residues were identified and proline was predicted as the most effective replacement amino acid. Pru p 3 C1 was produced in E.coli using a pET-based expression system. After refolding and purification by cation exchange chromatography, the protein was characterized by r...
متن کاملHypoallergenic allergen derivatives of Pru p 3 for immunotherapy of IgE-mediated peach allergy
Background IgE-mediated allergy to peach represents a frequent cause of severe anaphylactic reactions in Mediterranean countries. Pru p 3, a nonspecific lipid transfer protein (LTP) from peach, was identified as an important elicitor of IgEmediated food hypersensitivity reactions to peach. Specific immunotherapy is currently the only allergen-specific and disease-modifying treatment of allergie...
متن کاملPru p 3-sensitised Italian peach-allergic patients are less likely to develop severe symptoms when also presenting IgE antibodies to Pru p 1 and Pru p 4.
BACKGROUND The roles played by different peach allergens with respect to symptom severity have not been completely ascertained. We have evaluated the diagnostic efficacy of peach recombinant allergens ImmunoCAP compared to peach in the identification of subjects at an increased risk for severe reactions to peaches. METHODS 148 peach-allergic patients were divided based on their symptom severi...
متن کاملImpact of glutathione on the allergenicity of the peach lipid transfer protein Pru p 3.
BACKGROUND AND OBJECTIVE The allergenic potential of proteins can be altered under various physicochemical conditions. Glutathione (GSH) is a reducing agent that is used as an antioxidant in food products. We aimed to characterize the natural folding of peach proteins and test the allergenicity of reduced and natural Pru p 3, the major peach allergen. METHODS Pru p 3 was purified from peach, ...
متن کاملFruit Quality Attributes of New Peach and Nectarine Varieties under Selection in the Ebro Valley Conditions (Spain)
A breeding program searching for new peach and nectarine varieties adapted to the Ebro Valley growing conditions is being developed at the Aula Dei Experimental Station (CSIC). This program evaluates 15 populations obtained by controlled crosses. Different agronomic parameters such as blooming date, yield, and ripening date were determined in these populations. Quality traits such as fruit type...
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ژورنال
عنوان ژورنال: Allergy
سال: 2019
ISSN: 0105-4538,1398-9995
DOI: 10.1111/all.14102